![]() 4-IHC classification of breast cancer subtypes in a large cohort of a clinical cancer registry: use in clinical routine for therapeutic decisions and its effect on survival. Inwald EC, Koller M, Klinkhammer-Schalke M, Zeman F, Hofstadter F, Gerstenhauer M, et al. Ki67 in breast cancer: prognostic and predictive potential. Yerushalmi R, Woods R, Ravdin PM, Hayes MM, Gelmon KA. ![]() Prognostic value of different cut-off levels of Ki-67 in breast cancer: a systematic review and meta-analysis of 64,196 patients. Ki-67 as prognostic marker in early breast cancer: a meta-analysis of published studies involving 12,115 patients. 2016 535:308–12.ĭe Azambuja E, Cardoso F, de Castro G, Colozza M, Mano MS, Durbecq V, et al. Ki-67 acts as a biological surfactant to disperse mitotic chromosomes. 2000 406:747–52.Ĭuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser I, et al. Molecular portraits of human breast tumours. Perou CM, Sorlie T, Eisen MB, van de Rijn M, Jeffrey SS, Rees CA, et al. The image analysis system can substitute for or support visual counting by a pathologist. The performance of 30-9 is equivalent to that of MIB-1 in Ki67 assessment of breast cancer. Ki67-positive rates showed a strong correlation between the image analytical values and the pathologist-counted median values ( r = 0.952). Between 30–9 and MIB-1, there was no significant difference of CV%, showing variabilities of Ki67-positive rates among pathologists. Ki67-positive rates by 30-9 showed a strong correlation with those by MIB-1 for all pathologists (pathologist #1: r = 0.985, pathologist #2: r = 0.987, pathologist #3: r = 0.982). In addition, the images of 30-9-stained slides were analyzed using the image analysis system, VENTANA Virtuoso. Three pathologists independently studied images of the counting areas to determine Ki67-positive rates. We scanned all the stained slides with Ventana iScan HT and selected the Ki67 counting areas based on morphological findings. The tissue sections were stained immunohistochemically with anti-Ki67 antibodies, 30-9 and MIB-1, as well as with hematoxylin and eosin for morphological analysis. MethodsĪ series of sequential tissue sections was prepared from formalin-fixed paraffin-embedded blocks of surgically resected breast cancer specimens from 50 patients. We aimed to validate the performance of anti-Ki67 antibody clone 30-9 by comparison with clone MIB-1 and to investigate utility of the image analysis system in Ki67 assessment using clinical breast cancer samples. I will follow the OpenSlide progress with interest, since we would likely have to employ a similar strategy in order to implement a reader ourselves.Although Ki67 has important clinical relevance in breast cancer, its assessment results vary according to assay due to differences in both analytical and interpretation processes. Looking at the issue you mentioned for OpenSlide, the addition of the requirement to perform global tile registration in the absence of metadata which indicates the exact tile positions would appear to add a significant amount of work on top of adding a new reader. We are dedicated to maintaining Bio-Formats, but also, in the best spirit of open source development, sourcing contributions from the community for new formats. We have had several additions of this kind over the past few years and we always welcome independent community contributions to Bio-Formats as well ( ). On the other hand, we will review any external contribution with new reader additions and consider their inclusion in a minor release of Bio-Formats. While we certainly want to support as many formats as possible, the seemingly eternal development of new formats is hard to prioritise, given other pressing needs. ple-funded), which do fund cross-platform resources for writing OME-TIFF ( ) and support for several binary formats (BDV, etc.). Our priorities are necessarily our current academic deliverables (. ![]() We certainly have a growing backlog of many tens of new file formats, and Ventana BIF is amongst these - ĭeveloping and testing new file format readers is significantly more costly than maintaining existing ones. Unfortunately this particular format is not yet supported. ![]()
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